The high prevalence of Clostridioides difficile among nursing home elders associates with a dysbiotic microbiome.

ABSTRACT: Clostridioides difficile disproportionally affects the elderly living in nursing homes (NHs). Our objective was to explore the prevalence of C. difficile in NH elders, over time and to determine whether the microbiome or other clinical factors are associated with C. difficile colonization.We collected serial stool samples from NH residents. C. difficile prevalence was determined by quantitative polymerase-chain reaction detection of Toxin genes tcdA and tcdB; microbiome composition was determined by shotgun metagenomic sequencing. We used mixed-effect random forest modeling machine to determine bacterial taxa whose abundance is associated with C. difficile prevalence while controlling for clinical covariates including demographics, medicatio

SUBMITTER: Haran JP 

PROVIDER: S-EPMC8007149 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature