TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell.

ABSTRACT:

Background

Oxaliplatin is the first-choice chemotherapy method for patients with advanced colon cancer. However, its resistance leads to treatment failure for many patients. In our experiments, we aim to elucidate the associations among TRIM29 protein, mutant P53, and the resistance of colon cancer cells to oxaliplatin.

Methods

HCT116 and HT-29 cells were cultured and transfected with plasmids pIRES2-ZsGreen1-TRIM29-flag. Western blot and real-time qRT-PCR were utilized to examine the protein and mRNA expressions of TRIM29 and other related markers, respectively. MTT assay was utilized to determine the cell growth rate and generate the inhibition curve. Continuous culture in low-concentration oxaliplatin was conducted to construct oxaliplatin-resistant cell lines. The coimmu

SUBMITTER: Lei G 

PROVIDER: S-EPMC8007381 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature