In vitro skin culture media influence the viability and inflammatory response of primary macrophages.

ABSTRACT: The replacement of animal models for investigation of inflammation and wound healing has been advancing by means of in vitro skin equivalents with increasing levels of complexity. However, the current in vitro skin models still have a limited pre-clinical relevance due to their lack of immune cells. So far, few steps have been made towards the incorporation of immune cells into in vitro skin and the requirements for immunocompetent co-cultures remain unexplored. To establish suitable conditions for incorporating macrophages into skin models, we evaluated the effects of different media on primary keratinocytes, fibroblasts and macrophages. Skin maturation was affected by culture in macrophage medium, while macrophages showed reduced viability, altered cell morphology and decreased response

SUBMITTER: Griffoni C 

PROVIDER: S-EPMC8007571 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature