Polyubiquitin and ubiquitin-like signals share common recognition sites on proteasomal subunit Rpn1.

ABSTRACT: Proteasome-mediated substrate degradation is an essential process that relies on the coordinated actions of ubiquitin (Ub), shuttle proteins containing Ub-like (UBL) domains, and the proteasome. Proteinaceous substrates are tagged with polyUb and shuttle proteins, and these signals are then recognized by the proteasome, which subsequently degrades the substrate. To date, three proteasomal receptors have been identified, as well as multiple shuttle proteins and numerous types of polyUb chains that signal for degradation. While the components of this pathway are well-known, our understanding of their interplay is unclear-especially in the context of Rpn1, the largest proteasomal subunit. Here, using nuclear magnetic resonance (NMR) spectroscopy in combination with competition assays, we show

SUBMITTER: Boughton AJ 

PROVIDER: S-EPMC8008175 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature