Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis.

ABSTRACT:

Background

We report a method to estimate carbon assimilation based on isotope ratio-mass spectrometry (IRMS) of ¹³CO₂ labeled plant tissue. Photosynthetic carbon assimilation is the principal experimental observable which integrates important aspects of primary plant metabolism. It is traditionally measured through gas exchange. Despite its centrality in plant research, gas exchange performs poorly with rosette growth habits typical of Arabidopsis thaliana, mutant lines with limited biomass, and accounts poorly for leaf shading.

Results

IRMS-based carbon assimilation values from plants labeled at different light intensities were compared to those obtained by gas exchange, and the two methods yielded similar values. Using this method, we observed a stro

SUBMITTER: Bergman ME 

PROVIDER: S-EPMC8008545 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature