Persistence and dynamics of fluorescent Lactobacillus plantarum in the healthy versus inflamed gut.

ABSTRACT: The gastrointestinal tract is the main ecological niche in which Lactobacillus strains may provide health benefits in mammals. There is currently a need to characterize host-microbe interactions in space and time by tracking these bacteria in vivo. We combined noninvasive whole-body imaging with ex vivo fluorescence confocal microscopy imaging to monitor the impact of intestinal inflammation on the persistence of orally administered Lactobacillus plantarum NCIMB8826 in healthy and inflamed mouse colons. We developed fluorescent L. plantarum strains and demonstrated that mCherry is the best system for in vivo imaging and ex vivo fluorescence confocal microscopy of these bacteria. We also used whole-body imaging to show that this anti-inflamma

SUBMITTER: Salome-Desnoulez S 

PROVIDER: S-EPMC8009120 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature