Alteration of N ⁶ -Methyladenosine mRNA Methylation in a Rat Model of Cerebral Ischemia-Reperfusion Injury.

ABSTRACT:

Aim

This study was conducted in order to reveal the alterations in the N ⁶-methyladenosine (m6A) modification profile of cerebral ischemia-reperfusion injury model rats.

Materials and methods

Rats were used to establish the middle cerebral artery occlusion and reperfusion (MCAO/R) model. MeRIP-seq and RNA-seq were performed to identify differences in m6A methylation and gene expression. The expression of m6A methylation regulators was analyzed in three datasets and detected by quantitative real-time polymerase chain reaction, western blot, and immunofluorescence.

Results

We identified 1,160 differentially expressed genes with hypermethylated or hypomethylated m6A modifications. The differentially expressed genes with hypermethylated m6A modifications

SUBMITTER: Yi D 

PROVIDER: S-EPMC8009187 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature