Reporter system architecture affects measurements of noncanonical amino acid incorporation efficiency and fidelity.

ABSTRACT: The ability to genetically encode noncanonical amino acids (ncAAs) within proteins supports a growing number of applications ranging from fundamental biological studies to enhancing the properties of biological therapeutics. Currently, our quantitative understanding of ncAA incorporation systems is confounded by the diverse set of characterization and analysis approaches used to quantify ncAA incorporation events. While several effective reporter systems support such measurements, it is not clear how quantitative results from different reporters relate to one another, or which details influence measurements most strongly. Here, we evaluate the quantitative performance of single-fluorescent protein reporters, dual-fluorescent protein reporters, and cell surface-displayed protein reporters o

SUBMITTER: Potts KA 

PROVIDER: S-EPMC8009230 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature