Phagocytosis of apoptotic endothelial cells reprograms macrophages in skin wounds.

ABSTRACT: In healing wounds, the regression of blood vessels during the resolution phase creates a significant number of apoptotic endothelial cells (ApoECs). Surprisingly few studies have investigated the fate of apoECs in wounds, or the consequence of their removal. The current study employed both in vitro and in vivo models to investigate if macrophages ingest apoECs and to determine if such phagocytosis alters macrophage phenotype. To examine the capability of macrophages to ingest apoECs in in vivo wounds, pHrodo green labeled apoECs were injected into skin wounds 6 days after injury. The results demonstrated that 2.2% of macrophages in the wounds had engulfed apoECs 24 hours after injection. Macrophages that had engulfed apoECs expressed the markers CD80 (100%), CD86 (93.8

SUBMITTER: Xu M 

PROVIDER: S-EPMC8009329 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature