Assessment of a Silicon-Photomultiplier-Based Platform for the Measurement of Intracellular Calcium Dynamics with Targeted Aequorin.

ABSTRACT: Ca²⁺ is among the most important intracellular second messengers participating in a plethora of biological processes, and the measurement of Ca²⁺ fluctuations is significant in the phenomenology of the underlying processes. Aequorin-based Ca²⁺ probes represent an invaluable tool for reliable measurement of Ca²⁺ concentrations and dynamics in different subcellular compartments. However, their use is limited due to the lack on the market of ready-to-use, cost-effective, and portable devices for the detection and readout of the low-intensity bioluminescence signal produced by these probes. Silicon photomultipliers (SiPMs) are rapidly evolving solid-state sensors for low light detection, with single photon sensitivity and photon number resolving capa

SUBMITTER: Ruffinatti FA 

PROVIDER: S-EPMC8009593 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature