Cuprizone and EAE mouse frontal cortex proteomics revealed proteins altered in multiple sclerosis.

ABSTRACT: Two pathophysiological different experimental models for multiple sclerosis were analyzed in parallel using quantitative proteomics in attempts to discover protein alterations applicable as diagnostic-, prognostic-, or treatment targets in human disease. The cuprizone model reflects de- and remyelination in multiple sclerosis, and the experimental autoimmune encephalomyelitis (EAE, MOG1-125) immune-mediated events. The frontal cortex, peripheral to severely inflicted areas in the CNS, was dissected and analyzed. The frontal cortex had previously not been characterized by proteomics at different disease stages, and novel protein alterations involved in protecting healthy tissue and assisting repair of inflicted areas might be discovered. Using TMT-labelling and mass spectrometry, 1871 of th

SUBMITTER: Oveland E 

PROVIDER: S-EPMC8010076 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature