Nanoscale microenvironment engineering for expanding human hair follicle stem cell and revealing their plasticity.

ABSTRACT:

Background

Periodically regenerated hair follicles provide an excellent research model for studying tissue regeneration and stem cell homeostasis. Periodic activation and differentiation of hair follicle stem cells (HFSCs) fuel cyclical bouts of hair regeneration. HFSCs represent an excellent paradigm for studying tissue regeneration and somatic stem cell homeostasis. However, these crucial studies are hampered by the lack of a culture system able to stably expand human HFSCs and regulate their fate.

Results

Here, we use layer-by-layer (LbL) self-assembly with gelatin/alginate to construct a nanoscale biomimetic extracellular matrix (ECM) for an HFSC population. The LbL coating provides ECM and mechanical support for individual cells, which helps to maintain the CD200^+<

SUBMITTER: Chen P 

PROVIDER: S-EPMC8010974 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature