Bio-guided isolation of anti-leishmanial natural products from Diospyros gracilescens L. (Ebenaceae).

ABSTRACT:

Background

Plants represent an intricate and innovative source for the discovery of novel therapeutic remedies for the management of infectious diseases. The current study aimed at discovering new inhibitors of Leishmania spp., using anti-leishmanial activity-guided investigation approach of extracts from Diospyros gracilescens Gürke (1911) (Ebenaceae), targeting the extracellular (promastigotes) and intracellular (amastigotes) forms of Leishmania donovani.

Methods

The plant extracts were prepared by maceration using H₂0: EtOH (30:70, v/v) and further fractionated using a bio-guided approach. Different concentrations of D. gracilescens extracts, fractions and isolated compounds were tested in triplicate against L. donovani promastigotes and amastigotes in vitro. T

SUBMITTER: Njanpa CAN 

PROVIDER: S-EPMC8011081 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature