The Expression and Purification of Recombinant Mouse Ameloblastin in E. coli.
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ABSTRACT:
SUBMITTER: Su J
PROVIDER: S-EPMC8011534 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
The Expression and Purification of Recombinant Mouse Ameloblastin in E. coli.
Su Jingtan J Bapat Rucha Arun RA Moradian-Oldak Janet J
Methods in molecular biology (Clifton, N.J.) 20190101
Ameloblastin is the second most abundant enamel matrix protein, and is thought to be essential for ameloblast cell polarization, cell adhesion, and enamel mineralization. However, studies of ameloblastin's function and its molecular mechanism have been limited due to difficulty in obtaining recombinant ameloblastin in vitro. Here, we present a protocol for successful ameloblastin expression and purification in E. coli. ...[more]
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