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Laminin-111 Improves the Anabolic Response to Mechanical Load in Aged Skeletal Muscle.

ABSTRACT: Anabolic resistance to a mechanical stimulus may contribute to the loss of skeletal muscle mass observed with age. In this study, young and aged mice were injected with saline or human LM-111 (1 mg/kg). One week later, the myotendinous junction of the gastrocnemius muscle was removed via myotenectomy (MTE), thus placing a chronic mechanical stimulus on the remaining plantaris muscle for 2 weeks. LM-111 increased α7B integrin protein expression and clustering of the α7B integrin near DAPI+ nuclei in aged muscle in response to MTE. LM-111 reduced CD11b+ immune cells, enhanced repair, and improved the growth response to loading in aged plantaris muscle. These results suggest that LM-111 may represent a novel therapeutic approach to prevent and/or treat sarcopenia.

SUBMITTER: Garg K

PROVIDER: S-EPMC8011696 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Laminin-111 Improves the Anabolic Response to Mechanical Load in Aged Skeletal Muscle.

Garg Koyal K Mahmassani Ziad S ZS Dvoretskiy Svyatoslav S Valero M Carmen MC Huntsman Heather D HD Lapp Samuel S Wu Yu-Fu YF Hauschka Stephen D SD Burkin Dean J DJ Boppart Marni D MD

The journals of gerontology. Series A, Biological sciences and medical sciences 20210301 4

Anabolic resistance to a mechanical stimulus may contribute to the loss of skeletal muscle mass observed with age. In this study, young and aged mice were injected with saline or human LM-111 (1 mg/kg). One week later, the myotendinous junction of the gastrocnemius muscle was removed via myotenectomy (MTE), thus placing a chronic mechanical stimulus on the remaining plantaris muscle for 2 weeks. LM-111 increased α7B integrin protein expression and clustering of the α7B integrin near DAPI+ nuclei ...[more]

PMID: 33284954

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