HPV-Associated Tumor Eradication by Vaccination with Synthetic Short Peptides and Particle-Forming Liposomes.

ABSTRACT: Human papilloma virus (HPV)-16 is associated with cervical cancers and induces expression of the E6 and E7 oncogenes. Using a murine cell line that expresses these, the genes are sequenced, and six predicted major histocompatibility complex (MHC) class I (MHC-I) epitopes are identified. A liposomal vaccine adjuvant based on cobalt-porphyrin-phospholipid (CoPoP) is admixed with synthetic 9-mer epitopes appended with three histidine residues, resulting in rapid formation of peptide-liposome particles. Immunization with multivalent peptides leads to protection from tumor challenge. Of the peptides screened, only the previously identified E7_49-57 epitope is functional. The peptide-liposome particles that form upon mixing E7_HHH49-57 with CoPoP liposomes are stable in serum

SUBMITTER: He X 

PROVIDER: S-EPMC8011812 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature