NMDAR-independent, cAMP-dependent antidepressant actions of ketamine.

ABSTRACT: Ketamine produces rapid and robust antidepressant effects in depressed patients within hours of administration, often when traditional antidepressant compounds have failed to alleviate symptoms. We hypothesized that ketamine would translocate Gα_s from lipid rafts to non-raft microdomains, similarly to other antidepressants but with a distinct, abbreviated treatment duration. C6 glioma cells were treated with 10 µM ketamine for 15 min, which translocated Gα_s from lipid raft domains to non-raft domains. Other NMDA antagonist did not translocate Gα_s from lipid raft to non-raft domains. The ketamine-induced Gα_s plasma membrane redistribution allows increased functional coupling of Gα_s and adenylyl cyclase to increase intracellular cyclic a

SUBMITTER: Wray NH 

PROVIDER: S-EPMC8011999 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature