Aedes aegypti miRNA-33 modulates permethrin induced toxicity by regulating VGSC transcripts.

ABSTRACT: Aedes aegypti is a major vector of Zika, dengue, and other arboviruses. Permethrin adulticidal spraying, which targets the voltage-gated sodium channel (VGSC), is commonly done to reduce local mosquito populations and protect humans from exposure to arbovirus pathogens transmitted by this dangerous pest. Permethrin resistance, however, is a growing problem and understanding its underlying molecular basis may identify avenues to combat it. We identified a single G:C polymorphism in pre-miR-33 that was genetically associated with permethrin resistance; resulting isoforms had structural differences that may affect DICER-1/pre-miRNA processing rates. We then assessed the effects of overexpression of pre-miR-33 isoforms on permethrin toxicological phenotypes, VGSC transcript abundance and prote

SUBMITTER: Kubik TD 

PROVIDER: S-EPMC8012613 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature