EDNA metabarcoding of small plankton samples to detect fish larvae and their preys from Atlantic and Pacific waters.

ABSTRACT: Zooplankton community inventories are the basis of fisheries management for containing fish larvae and their preys; however, the visual identification of early-stage larvae (the "missing biomass") is difficult and laborious. Here, eDNA metabarcoding was employed to detect zooplankton species of interest for fisheries from open and coastal waters. High-Throughput sequencing (HTS) from environmental samples using small water volumes has been proposed to detect species of interest whose DNA is the most abundant. We analyzed 6-L water samples taken from subtropical and tropical waters using Cytochrome oxidase I (COI) gene as metabarcode. In the open ocean, several commercial fish larvae and invertebrate species important in fish diet were found from metabarcodes and confirmed from individual b

SUBMITTER: Garcia-Vazquez E 

PROVIDER: S-EPMC8012656 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature