A streamlined whole blood CyTOF workflow defines a circulating immune cell signature of COVID-19.

ABSTRACT: Mass cytometry (CyTOF) represents one of the most powerful tools in immune phenotyping, allowing high throughput quantification of over 40 parameters at single-cell resolution. However, wide deployment of CyTOF-based immune phenotyping studies are limited by complex experimental workflows and the need for specialized CyTOF equipment and technical expertise. Furthermore, differences in cell isolation and enrichment protocols, antibody reagent preparation, sample staining, and data acquisition protocols can all introduce technical variation that can confound integrative analyses of large data-sets of samples processed across multiple labs. Here, we present a streamlined whole blood CyTOF workflow which addresses many of these sources of experimental variation and facilitates wider adoption o

SUBMITTER: Geanon D 

PROVIDER: S-EPMC8013522 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature