Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis II.

ABSTRACT: Partitioning of the genome in meiosis occurs through two highly specialized cell divisions, named meiosis I and meiosis II. Step-wise cohesin removal is required for chromosome segregation in meiosis I, and sister chromatid segregation in meiosis II. In meiosis I, mono-oriented sister kinetochores appear as fused together when examined by high-resolution confocal microscopy, whereas they are clearly separated in meiosis II, when attachments are bipolar. It has been proposed that bipolar tension applied by the spindle is responsible for the physical separation of sister kinetochores, removal of cohesin protection, and chromatid separation in meiosis II. We show here that this is not the case, and initial separation of sister kinetochores occurs already in anaphase I independently of bipolar

SUBMITTER: Gryaznova Y 

PROVIDER: S-EPMC8013791 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature