High-Resolution DNA Dual-Rulers Reveal a New Intermediate State in Ribosomal Frameshifting.

ABSTRACT: Ribosomal frameshifting is an important pathway used by many viruses for protein synthesis that involves mRNA translocation of various numbers of nucleotides. Resolving the mRNA positions with subnucleotide precision will provide critical mechanistic information that is difficult to obtain with current techniques. We report a method of high-resolution DNA rulers with subnucleotide precision and the discovery of new frameshifting intermediate states on mRNA containing a GA₇ G motif. Two intermediate states were observed with the aid of fusidic acid, one at the "0" reading frame and the other near the "-1" reading frame, in contrast to the "-2" and "-1" frameshifting products found in the absence of the antibiotic. We termed the new near-"-1" intermediate the Post(-1*) state becau

SUBMITTER: Mao Y 

PROVIDER: S-EPMC8014572 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature