Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in serum samples of African origin.

ABSTRACT:

Objectives

Specific serological tests are mandatory for reliable SARS-CoV-2 diagnostics and seroprevalence studies. Here, we assess the specificities of four commercially available SARS-CoV-2 IgG ELISAs in serum/plasma panels originating from Africa, South America, and Europe.

Methods

882 serum/plasma samples collected from symptom-free donors before the COVID-19 pandemic in three African countries (Ghana, Madagascar, Nigeria), Colombia, and Germany were analysed with three nucleocapsid-based ELISAs (Euroimmun Anti-SARS-CoV-2-NCP IgG, EDI™ Novel Coronavirus COVID-19 IgG, Mikrogen recomWell SARS-CoV-2 IgG), one spike/S1-based ELISA (Euroimmun Anti-SARS-CoV-2 IgG), and in-house common cold CoV ELISAs.

Results

High specificity was confirmed for all SARS-CoV-2 IgG ELISAs

SUBMITTER: Emmerich P 

PROVIDER: S-EPMC8014856 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature