The Mouse Heart Mitochondria N Terminome Provides Insights into ClpXP-Mediated Proteolysis.

ABSTRACT: The mammalian mitochondrial proteome consists of more than 1100 annotated proteins and their proteostasis is regulated by only a few ATP-dependent protease complexes. Technical advances in protein mass spectrometry allowed for detailed description of the mitoproteome from different species and tissues and their changes under specific conditions. However, protease-substrate relations within mitochondria are still poorly understood. Here, we combined Terminal Amine Isotope Labeling of Substrates (TAILS) N termini profiling of heart mitochondria proteomes isolated from wild type and Clpp^-/- mice with a classical substrate-trapping screen using FLAG-tagged proteolytically active and inactive CLPP variants to identify new ClpXP substrates in mammalian mitochondria. Using TAILS

SUBMITTER: Hofsetz E 

PROVIDER: S-EPMC8014998 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature