Molecular Dynamics Simulation-assisted Ionic Liquid Screening for Deep Coverage Proteome Analysis.

ABSTRACT: In-depth coverage of proteomic analysis could enhance our understanding to the mechanism of the protein functions. Unfortunately, many highly hydrophobic proteins and low-abundance proteins, which play critical roles in signaling networks, are easily lost during sample preparation, mainly attributed to the fact that very few extractants can simultaneously satisfy the requirements on strong solubilizing ability to membrane proteins and good enzyme compatibility. Thus, it is urgent to screen out ideal extractant from the huge compound libraries in a fast and effective way. Herein, by investigating the interior mechanism of extractants on the membrane proteins solubilization and trypsin compatibility, a molecular dynamics simulation system was established as complement to the experimental pro

SUBMITTER: Fang F 

PROVIDER: S-EPMC8015004 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature