Orbit/CLASP determines centriole length by antagonising Klp10A in Drosophila spermatocytes.

ABSTRACT: After centrosome duplication, centrioles elongate before M phase. To identify genes required for this process and to understand the regulatory mechanism, we investigated the centrioles in Drosophila premeiotic spermatocytes expressing fluorescently tagged centriolar proteins. We demonstrated that an essential microtubule polymerisation factor, Orbit (the Drosophila CLASP orthologue, encoded by chb), accumulated at the distal end of centrioles and was required for the elongation. Conversely, a microtubule-severing factor, Klp10A, shortened the centrioles. Genetic analyses revealed that these two proteins functioned antagonistically to determine centriole length. Furthermore, Cp110 in the distal tip complex was closely associated with the factors involved in centriolar d

SUBMITTER: Shoda T 

PROVIDER: S-EPMC8015252 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature