Viral manipulation of functionally distinct interneurons in mice, non-human primates and humans.

ABSTRACT: Recent success in identifying gene-regulatory elements in the context of recombinant adeno-associated virus vectors has enabled cell-type-restricted gene expression. However, within the cerebral cortex these tools are largely limited to broad classes of neurons. To overcome this limitation, we developed a strategy that led to the identification of multiple new enhancers to target functionally distinct neuronal subtypes. By investigating the regulatory landscape of the disease gene Scn1a, we discovered enhancers selective for parvalbumin (PV) and vasoactive intestinal peptide-expressing interneurons. Demonstrating the functional utility of these elements, we show that the PV-specific enhancer allowed for the selective targeting and manipulation of these neurons across vertebrate species, in

SUBMITTER: Vormstein-Schneider D 

PROVIDER: S-EPMC8015416 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature