Mutant p53 regulates Survivin to foster lung metastasis.

ABSTRACT: Esophageal squamous cell carcinoma (ESCC) is one of the most lethal cancers worldwide and evolves often to lung metastasis. P53^R175H (homologous to Trp53^R172H in mice) is a common hot spot mutation. How metastasis is regulated by p53^R175H in ESCC remains to be investigated. To investigate p53^R175H-mediated molecular mechanisms, we used a carcinogen-induced approach in Trp53^R172H/- mice to model ESCC. In the primary Trp53^R172H/- tumor cell lines, we depleted Trp53^R172H (shTrp53) and observed a marked reduction in cell invasion in vitro and lung metastasis burden in a tail-vein injection model in comparing isogenic cells (shCtrl). Furthermore, we performed bulk RNA-seq to compare g

SUBMITTER: Tang Q 

PROVIDER: S-EPMC8015716 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature