Human nucleoli comprise multiple constrained territories, tethered to individual chromosomes.
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ABSTRACT:
SUBMITTER: Mangan H
PROVIDER: S-EPMC8015717 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Publications
Human nucleoli comprise multiple constrained territories, tethered to individual chromosomes.
Genes & development 20210304 7-8
It is unknown how ribosomal gene (rDNA) arrays from multiple chromosomal nucleolar organizers (NORs) partition within human nucleoli. Exploration of this paradigm for chromosomal organization is complicated by the shared DNA sequence composition of five NOR-bearing acrocentric chromosome p-arms. Here, we devise a methodology for genetic manipulation of individual NORs. Efficient "scarless" genome editing of rDNA repeats is achieved on "poised" human NORs held within monochromosomal cell hybrids. ...[more]
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