Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device.

ABSTRACT: Myocardial infarction and heart failure are leading causes of death worldwide, in large part because adult human myocardium has extremely limited regeneration capacity. Zebrafish are a powerful model for identifying new strategies for human cardiac repair because their hearts regenerate after relatively severe injuries. Zebrafish are also relatively scalable and compatible with many genetic tools. However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque, preventing live imaging in vivo. An alternative strategy is to explant and culture intact adult zebrafish hearts and investigate them ex vivo. However, explanted hearts maintained in conventional culture conditions experience rapid declines in morphology and physio

SUBMITTER: Yip JK 

PROVIDER: S-EPMC8015799 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature