Calcium signaling in endocardial and epicardial ventricular myocytes from streptozotocin-induced diabetic rats.

ABSTRACT:

Aims/introduction

Abnormalities in Ca²⁺ signaling have a key role in hemodynamic dysfunction in diabetic heart. The purpose of this study was to explore the effects of streptozotocin (STZ)-induced diabetes on Ca²⁺ signaling in epicardial (EPI) and endocardial (ENDO) cells of the left ventricle after 5-6 months of STZ injection.

Materials and methods

Whole-cell patch clamp was used to measure the L-type Ca²⁺ channel (LTCC) and Na⁺ /Ca²⁺ exchanger currents. Fluorescence photometry techniques were used to measure intracellular free Ca²⁺ concentration.

Results

Although the LTCC current was not significantly altered, the amplitude of Ca²⁺ transients increased significantly in EPI-STZ and ENDO-STZ

SUBMITTER: Al Kury LT 

PROVIDER: S-EPMC8015823 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature