Conformational Differences Are Observed for the Active and Inactive Forms of Pinholin S²¹ Using DEER Spectroscopy.

ABSTRACT: Bacteriophages have evolved with an efficient host cell lysis mechanism to terminate the infection cycle and release the new progeny virions at the optimum time, allowing adaptation with the changing host and environment. Among the lytic proteins, holin controls the first and rate-limiting step of host cell lysis by permeabilizing the inner membrane at an allele-specific time known as "holin triggering". Pinholin S²¹ is a prototype holin of phage Φ21 which makes many nanoscale holes and destroys the proton motive force, which in turn activates the signal anchor release (SAR) endolysin system to degrade the peptidoglycan layer of the host cell and destruction of the outer membrane by the spanin complex. Like many others, phage Φ21 has two holin proteins: active pinholin and antip

SUBMITTER: Ahammad T 

PROVIDER: S-EPMC8015962 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature