Accurate detection of Newcastle disease virus using proximity-dependent DNA aptamer ligation assays.

ABSTRACT: Detecting viral antigens at low concentrations in field samples can be crucial for early veterinary diagnostics. Proximity ligation assays (PLAs) in both solution and solid-phase formats are widely used for high-performance protein detection in medical research. However, the affinity reagents used, which are mainly poly- and monoclonal antibodies, play an important role in the performance of PLAs. Here, we have established the first homogeneous and solid-phase proximity-dependent DNA aptamer ligation assays for rapid and accurate detection of Newcastle disease virus (NDV). NDV is detected by a pair of extended DNA aptamers that, upon binding in proximity to proteins on the envelope of the virus, are joined by enzymatic ligation to form a unique amplicon that can be sensitively detected usi

SUBMITTER: Marnissi B 

PROVIDER: S-EPMC8016122 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature