A comprehensive metagenomics framework to characterize organisms relevant for planetary protection.

ABSTRACT:

Background

Clean rooms of the Space Assembly Facility (SAF) at the Jet Propulsion Laboratory (JPL) at NASA are the final step of spacecraft cleaning and assembly before launching into space. Clean rooms have stringent methods of air-filtration and cleaning to minimize microbial contamination for exoplanetary research and minimize the risk of human pathogens, but they are not sterile. Clean rooms make a selective environment for microorganisms that tolerate such cleaning methods. Previous studies have attempted to characterize the microbial cargo through sequencing and culture-dependent protocols. However, there is not a standardized metagenomic workflow nor analysis pipeline for spaceflight hardware cleanroom samples to identify microbial contamination. Additionally, current identi

SUBMITTER: Danko DC 

PROVIDER: S-EPMC8016160 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature