Farnesoid X receptor promotes renal ischaemia-reperfusion injury by inducing tubular epithelial cell apoptosis.

ABSTRACT:

Purpose

We investigated the role of farnesoid X receptor (FXR), a ligand-dependent transcription factor, in renal ischaemia-reperfusion (I/R) injury.

Materials and methods

We performed unilateral renal I/R model in FXR knockout (Fxr^-/- ) and wild-type (WT) mice in vivo and a hypoxia-reoxygenation (H/R) model in vitro. The pathways by which FXR induces apoptosis were detected using a proteome profiler array. The effects of FXR on apoptosis were evaluated using immunoblotting, TUNEL assays and flow cytometry.

Results

Compared with WT mice, Fxr^-/- mice showed improved renal function and reduced tubular injury scores and apoptosis. Consistent with the in vivo results, the silencing of FXR decreased the number of apoptotic HK-2 cells after H/R, while

SUBMITTER: Xu Y 

PROVIDER: S-EPMC8016637 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature