Cell-extracellular matrix interactions in the fluidic phase direct the topology and polarity of self-organized epithelial structures.

ABSTRACT:

Introduction

In vivo, cells are surrounded by extracellular matrix (ECM). To build organs from single cells, it is generally believed that ECM serves as scaffolds to coordinate cell positioning and differentiation. Nevertheless, how cells utilize cell-ECM interactions for the spatiotemporal coordination to different ECM at the tissue scale is not fully understood.

Methods

Here, using in vitro assay with engineered MDCK cells expressing H2B-mCherry (nucleus) and gp135/Podocalyxin-GFP (apical marker), we show in multi-dimensions that such coordination for epithelial morphogenesis can be determined by cell-soluble ECM interaction in the fluidic phase.

Results

The coordination depends on the native topology of ECM components such as sheet-like basement membrane (BM) and t

SUBMITTER: Ouyang M 

PROVIDER: S-EPMC8016639 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature