DNA-driven condensation assembles the meiotic DNA break machinery.

ABSTRACT: The accurate segregation of chromosomes during meiosis-which is critical for genome stability across sexual cycles-relies on homologous recombination initiated by DNA double-strand breaks (DSBs) made by the Spo11 protein^1,2. The formation of DSBs is regulated and tied to the elaboration of large-scale chromosome structures^3-5, but the protein assemblies that execute and control DNA breakage are poorly understood. Here we address this through the molecular characterization of Saccharomyces cerevisiae RMM (Rec114, Mei4 and Mer2) proteins-essential, conserved components of the DSB machinery². Each subcomplex of Rec114-Mei4 (a 2:1 heterotrimer) or Mer2 (a coiled-coil-containing homotetramer) is monodispersed in solution, but they independently condense with DNA

SUBMITTER: Claeys Bouuaert C 

PROVIDER: S-EPMC8016751 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature