DNMT3.1 controls trade-offs between growth, reproduction, and life span under starved conditions in Daphnia magna.

ABSTRACT: The cladoceran crustacean Daphnia has long been a model of energy allocation studies due to its important position in the trophic cascade of freshwater ecosystems. However, the loci for controlling energy allocation between life history traits still remain unknown. Here, we report CRISPR/Cas-mediated target mutagenesis of DNA methyltransferase 3.1 (DNMT3.1) that is upregulated in response to caloric restriction in Daphnia magna. The resulting biallelic mutant is viable and did not show any change in growth rate, reproduction, and longevity under nutrient rich conditions. In contrast, under starved conditions, the growth rate of this DNMT3.1 mutant was increased but its reproduction was reciprocally reduced compared to the wild type when the growth and reproduction activities competed durin

SUBMITTER: Nguyen ND 

PROVIDER: S-EPMC8016896 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature