Biosyntheses characterization of alkaloids and flavonoids in Sophora flavescens by combining metabolome and transcriptome.

ABSTRACT: Sophora flavescens are widely used for their pharmacological effects. As its main pharmacological components, alkaloids and flavonoids are distributed in the root tissues wherein molecular mechanisms remain elusive. In this study, metabolite profiles are analyzed using metabolomes to obtain biomarkers detected in different root tissues. These biomarkers include alkaloids, phenylpropanoids, and flavonoids. The high-performance liquid chromatography analysis results indicate the differences in principal component contents. Oxymatrine, sophoridine, and matrine contents are the highest in the phloem, whereas trifolirhizin, maackiain, and kushenol I contents are the highest in the xylem. The transcript expression profiles also show tissue specificity in the roots. A total of 52 and 39 transcrip

SUBMITTER: Wei G 

PROVIDER: S-EPMC8016917 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature