Transcriptome and IgH Repertoire Analyses Show That CD11c^hi B Cells Are a Distinct Population With Similarity to B Cells Arising in Autoimmunity and Infection.

ABSTRACT: A distinct B cell population marked by elevated CD11c expression is found in patients with systemic lupus erythematosus (SLE). Cells with a similar phenotype have been described during chronic infection, but variable gating strategies and nomenclature have led to uncertainty of their relationship to each other. We isolated CD11c^hi cells from peripheral blood and characterized them using transcriptome and IgH repertoire analyses. Gene expression data revealed the CD11c^hi IgD⁺ and IgD^- subsets were highly similar to each other, but distinct from naive, memory, and plasma cell subsets. Although CD11c^hi B cells were enriched in some germinal center (GC) transcripts and expressed numerous negative regulators of B cell receptor (BCR) activat

SUBMITTER: Maul RW 

PROVIDER: S-EPMC8017342 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature