Targeted Single-Cell RNA-seq Identifies Minority Cell Types of Kidney Distal Nephron.

ABSTRACT:

Background

Proximal tubule cells dominate the kidney parenchyma numerically, although less abundant cell types of the distal nephron have disproportionate roles in water and electrolyte balance.

Methods

Coupling of a FACS-based enrichment protocol with single-cell RNA-seq profiled the transcriptomes of 9099 cells from the thick ascending limb (CTAL)/distal convoluted tubule (DCT) region of the mouse nephron.

Results

Unsupervised clustering revealed Slc12a3 ⁺/Pvalb ⁺ and Slc12a3 ⁺/Pvalb ^- cells, identified as DCT1 and DCT2 cells, respectively. DCT1 cells appear to be heterogeneous, with orthogonally variable expression of Slc8a1, Calb1, and Ckb. An additional DCT1 subcluster sh

SUBMITTER: Chen L 

PROVIDER: S-EPMC8017539 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature