A constitutively monomeric UVR8 photoreceptor confers enhanced UV-B photomorphogenesis.

ABSTRACT: The plant ultraviolet-B (UV-B) photoreceptor UVR8 plays an important role in UV-B acclimation and survival. UV-B absorption by homodimeric UVR8 induces its monomerization and interaction with the E3 ubiquitin ligase COP1, leading ultimately to gene expression changes. UVR8 is inactivated through redimerization, facilitated by RUP1 and RUP2. Here, we describe a semidominant, hyperactive allele, namely uvr8-17D, that harbors a glycine-101 to serine mutation. UVR8^G101S overexpression led to weak constitutive photomorphogenesis and extreme UV-B responsiveness. UVR8^G101S was observed to be predominantly monomeric in vivo and, once activated by UV-B, was not efficiently inactivated. Analysis of a UVR8 crystal structure containing the G101S mutation revealed the disto

SUBMITTER: Podolec R 

PROVIDER: S-EPMC8017708 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature