Stress granule formation, disassembly, and composition are regulated by alphavirus ADP-ribosylhydrolase activity.

ABSTRACT: While biomolecular condensates have emerged as an important biological phenomenon, mechanisms regulating their composition and the ways that viruses hijack these mechanisms remain unclear. The mosquito-borne alphaviruses cause a range of diseases from rashes and arthritis to encephalitis, and no licensed drugs are available for treatment or vaccines for prevention. The alphavirus virulence factor nonstructural protein 3 (nsP3) suppresses the formation of stress granules (SGs)-a class of cytoplasmic condensates enriched with translation initiation factors and formed during the early stage of infection. nsP3 has a conserved N-terminal macrodomain that hydrolyzes ADP-ribose from ADP-ribosylated proteins and a C-terminal hypervariable domain that binds the essential SG component G3BP1. Here, w

SUBMITTER: Jayabalan AK 

PROVIDER: S-EPMC8017970 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature