Tumor cell and immune cell profiles in primary human glioblastoma: Impact on patient outcome.

ABSTRACT: The distribution and role of tumor-infiltrating leucocytes in glioblastoma (GBM) remain largely unknown. Here, we investigated the cellular composition of 55 primary (adult) GBM samples by flow cytometry and correlated the tumor immune profile with patient features at diagnosis and outcome. GBM single-cell suspensions were stained at diagnosis (n = 44) and recurrence following radiotherapy and chemotherapy (n = 11) with a panel of 8-color monoclonal antibody combinations for the identification and enumeration of (GFAP⁺ CD45^- ) tumor and normal astrocytic cells, infiltrating myeloid cells -i.e. microglial and blood-derived tumor-associated macrophages (TAM), M1-like, and M2-like TAM, neutrophils. and myeloid-derived suppressor cells (MDSC)- and tumor-infiltrating lymph

SUBMITTER: Gonzalez-Tablas Pimenta M 

PROVIDER: S-EPMC8018082 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature