VirION2: a short- and long-read sequencing and informatics workflow to study the genomic diversity of viruses in nature.

ABSTRACT: Microbes play fundamental roles in shaping natural ecosystem properties and functions, but do so under constraints imposed by their viral predators. However, studying viruses in nature can be challenging due to low biomass and the lack of universal gene markers. Though metagenomic short-read sequencing has greatly improved our virus ecology toolkit-and revealed many critical ecosystem roles for viruses-microdiverse populations and fine-scale genomic traits are missed. Some of these microdiverse populations are abundant and the missed regions may be of interest for identifying selection pressures that underpin evolutionary constraints associated with hosts and environments. Though long-read sequencing promises complete virus genomes on single reads, it currently suffers from high DNA requir

SUBMITTER: Zablocki O 

PROVIDER: S-EPMC8018248 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature