A novel method of high-purity extracellular vesicle enrichment from microliter-scale human serum for proteomic analysis.

ABSTRACT: We have developed a rapid, low-cost, and simple separation strategy to separate extracellular vesicles (EVs) from a small amount of serum (i.e.,<100 μL) with minimal contamination by serum proteins and lipoprotein particles to meet the high purity requirement for EV proteome analysis. EVs were separated by a novel polyester capillary channel polymer (PET C-CP) fiber phase/hydrophobic interaction chromatography (HIC) method which is rapid and can process small size samples. The collected EV fractions were subjected to a post-column cleanup protocol using a centrifugal filter to perform buffer exchange and eliminate potential coeluting non-EV proteins while minimizing EV sample loss. Downstream characterization demonstrated that our current strategy can separate EVs with the anticipated exos

SUBMITTER: Ji X 

PROVIDER: S-EPMC8018574 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature