Natural autoantibodies to the gonadotropin-releasing hormone receptor in polycystic ovarian syndrome.

ABSTRACT:

Context

Polycystic ovarian syndrome (PCOS) is a complex disease with different subtypes and unclear etiology. Among the frequent comorbidities are autoimmune diseases, suggesting that autoantibodies (aAb) may be involved in PCOS pathogenesis.

Objective

As the gonadal axis often is dysregulated, we tested the hypothesis that aAb to the gonadotropin-releasing hormone receptor (GnRH-R) are of diagnostic value in PCOS.

Design

An in vitro assay for quantifying aAb to the GnRH-R (GnRH-R-aAb) was established by using a recombinant fusion protein of full-length human GnRH-R and firefly luciferase. A commercial rabbit antiserum to human GnRH-R was used for standardization. Serum samples of control subjects and different cohorts of European PCOS patients (n = 1051) were analyze

SUBMITTER: Sattler LM 

PROVIDER: S-EPMC8018624 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature