Self-aligned sequential lateral field non-uniformities over channel depth for high throughput dielectrophoretic cell deflection.

ABSTRACT: Dielectrophoresis (DEP) enables the separation of cells based on subtle subcellular phenotypic differences by controlling the frequency of the applied field. However, current electrode-based geometries extend over a limited depth of the sample channel, thereby reducing the throughput of the manipulated sample (sub-μL min-1 flow rates and <105 cells per mL). We present a flow through device with self-aligned sequential field non-uniformities extending laterally across the sample channel width (100 μm) that are created by metal patterned over the entire depth (50 μm) of the sample channel sidewall using a single lithography step. This enables single-cell streamlines to undergo progressive DEP deflection with minimal dependence on the cell starting position, its orientation versus the field a

SUBMITTER: Huang X 

PROVIDER: S-EPMC8019514 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature