Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit.

ABSTRACT: Lipid droplets (LDs) are bioactive organelles found within the cytosol of the most eukaryotic and some prokaryotic cells. LDs are composed of neutral lipids encased by a monolayer of phospholipids and proteins. Hepatic LD lipids, such as ceramides, and proteins are implicated in several diseases that cause hepatic steatosis. Although previous methods have been established for LD isolation, they require a time-consuming preparation of reagents and are not designed for the isolation of multiple subcellular compartments. We sought to establish a new protocol to enable the isolation of LDs, endoplasmic reticulum (ER), and lysosomes from a single mouse liver. Further, all reagents used in the protocol presented here are commercially available and require minimal reagent preparation without sacr

SUBMITTER: Brettschneider J 

PROVIDER: S-EPMC8020118 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature