Unknown

Dataset Information

0

Osthole Attenuates Macrophage Activation in Experimental Asthma by Inhibitingthe NF-ĸB/MIF Signaling Pathway.


ABSTRACT: Inhibition of activated macrophages is an alternative therapeutic strategy for asthma. We investigated whether a coumarin compound, osthole, isolated from Cnidium monnieri (L.) Cuss, alleviated macrophage activation in vivo and in vitro. Osthole could reduce expression of a marker of activated macrophages, cluster of differentiation (CD)206, in an ovalbumin-challenge model of asthma in mice. Osthole could also inhibit infiltration of inflammatory cells, collagen deposition and production of proinflammatory cytokines [interleukin (IL)-1β, tumor necrosis factor-ɑ, macrophage migration inhibitory factor (MIF)] in asthmatic mice. In vitro, expression of phosphorylated-IĸBɑ, MIF and M2 cytokines (Ym-1, Fizz-1, arginase-1) in IL-4-induced macrophages decreased upon exposure to the NF-ĸB inhibitor MG-132. In our short hairpin (sh)RNA-MIF-knockdown model, reduced expression of M2 cytokines was detected in the IL-4 + shRNA-MIF group. Osthole could attenuate the proliferation and migration of an IL-4-induced rat alveolar macrophages line (NR8383). Osthole could reduce IL-4-induced translocation of nuclear factor-kappa B (NF-ĸB) in NR8383 cells. Collectively, our results suggest that osthole ameliorates macrophage activation in asthma by suppressing the NF-ĸB/MIF signaling pathway, and might be a potential agent for treating asthma.

SUBMITTER: Li R 

PROVIDER: S-EPMC8020258 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

altmetric image

Publications

Osthole Attenuates Macrophage Activation in Experimental Asthma by Inhibitingthe NF-ĸB/MIF Signaling Pathway.

Li Ruyi R   Song Peng P   Tang Guofang G   Wei Jianghong J   Rao Lizong L   Ma Libing L   Jiang Ming M   Huang Jianwei J   Xu Qing Q   Wu Jingjie J   Lv Qian Q   Yao Dong D   Xiao Bo B   Huang Haiming H   Lei Liping L   Feng Juntao J   Mo Biwen B  

Frontiers in pharmacology 20210322


Inhibition of activated macrophages is an alternative therapeutic strategy for asthma. We investigated whether a coumarin compound, osthole, isolated from <i>Cnidium</i> <i>monnieri</i> (L.) Cuss, alleviated macrophage activation <i>in vivo</i> and <i>in vitro</i>. Osthole could reduce expression of a marker of activated macrophages, cluster of differentiation (CD)206, in an ovalbumin-challenge model of asthma in mice. Osthole could also inhibit infiltration of inflammatory cells, collagen depos  ...[more]

Similar Datasets

| S-EPMC10086758 | biostudies-literature
| S-EPMC11438018 | biostudies-literature
| S-EPMC7564842 | biostudies-literature
| S-EPMC5599518 | biostudies-literature
| S-EPMC7060131 | biostudies-literature
| S-EPMC10975068 | biostudies-literature
| S-EPMC3586206 | biostudies-literature
| S-EPMC9311149 | biostudies-literature
| S-EPMC2442287 | biostudies-literature
| S-EPMC8573605 | biostudies-literature